Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
IH |
|
| Nucleophosmin (NPM) is a ubiquitously expressed oestrogen-regulated nucleolar phosphoprotein frequently overexpressed in a variety of human malignancies. It is a multifunctional protein, modulating diverse molecular functions which include ribosome biogenesis and centrosome duplication etc. It also functions as a molecular chaperone that prevents proteins from aggregating in the crowded environment of the nucleolus. It also modulates, both positively and negatively, the activity of some tumor suppressor genes such as p53 and Arf, and it is upregulated in response to stress stimuli including DNADmageand hypoxia. It continuously shuttles between the nucleus and cytoplasm. The NPM gene which is mapped to human chromosome 5q35 is involved in several tumor-associated chromosome translocations, which have resulted in the formation of fusion proteins that retain the amino terminus of NPM, including NPM-ALK4, NPM-RAR5 and NPM-MLF1. The protein is directly involved in cancer pathogenesis, as the NPM1gene is mutated in a number of human haematopoietic malignancies, such as promyelocytic leukaemia, anaplastic large cell lymphoma, acute myeloid leukaemia, chronic myelogenous leukaemia and MDS. However its role in oncogenesis is controversial as NPM has been attributed with both oncogenic and tumor suppressive functions. | | | Catalog # | N7100-15C | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Skeletal muscle | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 11E300 | | Host | Mouse | | Source | Chicken | | Concentration | Not determined. | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Ascites | | Immunogen | GST fusion protein corresponding to N-terminus of nucleophosmin fused to 14aa of ALK protein. Cellular Localization: Cell membrane. | | Specificity | Recognizes Nucleophosmin. Species Crossreactivity: chicken, mouse, rat, xenopus. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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