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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleophosmin (NPM, B23, MGC104254, NO38, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Nucleophosmin/B23.2, Nucleoplasmin Family Member 1, NPM1, Numatrin, TRK Fused Gene)

Anti -Nucleophosmin (NPM, B23, MGC104254, NO38, Nucleolar Phosphoprotein B23, Nucleolar
Protein NO38, Nucleophosmin/B23.2, Nucleoplasmin Family Member 1, NPM1, Numatrin, TRK
Fused Gene)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E. This phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor alpha (RAR alpha). As a consequence, a fusion protein (60-70kD) is produced containing parts of the two genes. Monoclonal antibody reacting specifically with B23 is a useful tool for the study of the involvement of B23 in nuclear functions, cytoplasmic/nuclear trafficking, and centrosome duplication.
Catalog #N7100-20C
Cellular Localization Nuclear
Positive Control NIH3T3 whole cell lysate.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/m
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9A68
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA.
PurityPurified
ImmunogenFull length native protein (purified) (Rat). Epitope: The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.
SpecificityRecognizes both phosphorylated and unphosphorylated B23 molecule at ~37kD. Species Crossreactivity: Human, Mouse, Rat, bovine, canine, Donkey and Hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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