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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleophosmin (NPM), NT

Anti -Nucleophosmin (NPM), NT

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein (1). NPM is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. Data suggest that transcription factor YY1 may play a role in NPM gene expression (2). A chromosomal translocation associated with myelodysplastic syndrome and acute myeloid leukemia (AML) was found to rearrange part of the nucleophosmin (NPM) gene. Myeloid leukemia factor 1 (MLF1) binds NPM and studies have indicated that MLF1 is normally located in the cytoplasm, whereas NPM-MLF1 is targeted to the nucleus, with highest levels in the nucleolus. The nuclear/nucleolar localization of NPM-MLF1 mirrors that of NPM, indicating that NPM trafficking signals direct MLF1 to an inappropriate cellular compartment in myeloid leukemia cells (3)
Catalog #N7100-20D
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:200,000
Immunohistochemistry: 1:100-1:250
Flow cytometry: 1:40
Immunoprecipitation: 1:60
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E411
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human NPM. MW: 35/38kD (2 isoforms).
SpecificityRecognizes human NPM. Does not crossreact with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesNPM, Nucleolar phosphoprotein B23, Numatrin, Nucleolar protein NO38


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