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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH FC

Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein (1). NPM is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. Data suggest that transcription factor YY1 may play a role in NPM gene expression (2). A chromosomal translocation associated with myelodysplastic syndrome and acute myeloid leukemia (AML) was found to rearrange part of the nucleophosmin (NPM) gene. Myeloid leukemia factor 1 (MLF1) binds NPM and studies have indicated that MLF1 is normally located in the cytoplasm, whereas NPM-MLF1 is targeted to the nucleus, with highest levels in the nucleolus. The nuclear/nucleolar localization of NPM-MLF1 mirrors that of NPM, indicating that NPM trafficking signals direct MLF1 to an inappropriate cellular compartment in myeloid leukemia cells (3)
Catalog #	N7100-20D
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:200,000
	Immunohistochemistry: 1:100-1:250
	Flow cytometry: 1:40
	Immunoprecipitation: 1:60
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E411
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues on the N-terminus of human NPM. MW: 35/38kD (2 isoforms).
Specificity	Recognizes human NPM. Does not crossreact with mouse or rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	NPM, Nucleolar phosphoprotein B23, Numatrin, Nucleolar protein NO38