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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleophosmin (Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin, NPM1, NPM)

Anti -Nucleophosmin (Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin, NPM1,
NPM)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Nucleophosmin (NPM) is a ubiquitously expressed oestrogen-regulated nucleolar phosphoprotein frequently overexpressed in a variety of human malignancies. It is a multifunctional protein, modulating diverse molecular functions which include ribosome biogenesis and centrosome duplication etc. It also functions as a molecular chaperone that prevents proteins from aggregating in the crowded environment of the nucleolus. It also modulates, both positively and negatively, the activity of some tumor suppressor genes such as p53 and Arf, and it is upregulated in response to stress stimuli including DNADmageand hypoxia. It continuously shuttles between the nucleus and cytoplasm. The NPM gene which is mapped to human chromosome 5q35 is involved in several tumor-associated chromosome translocations, which have resulted in the formation of fusion proteins that retain the amino terminus of NPM, including NPM-ALK4, NPM-RAR5 and NPM-MLF1. The protein is directly involved in cancer pathogenesis, as the NPM1gene is mutated in a number of human haematopoietic malignancies, such as promyelocytic leukaemia, anaplastic large cell lymphoma, acute myeloid leukaemia, chronic myelogenous leukaemia and MDS. However its role in oncogenesis is controversial as NPM has been attributed with both oncogenic and tumor suppressive functions.
Catalog #N7100-20H
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman HeLa lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to CQEAIQDLWQWRKSL, from the C-terminus of the protein sequence according to NP_002511; NP_954654.
SpecificityRecognizes human Nucleophosmin/NPM1. Species sequence homology: chimpanzee, canine, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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