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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -NUP35 (Nucleoporin NUP53, 35kD Nucleoporin, Mitotic Phosphoprotein 44, MP-44, Nuclear Pore Complex Protein Nup53, Nucleoporin Nup35, MP44, NUP53)

Anti -NUP35 (Nucleoporin NUP53, 35kD Nucleoporin, Mitotic Phosphoprotein 44, MP-44,
Nuclear Pore Complex Protein Nup53, Nucleoporin Nup35, MP44, NUP53)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Nucleoporin Nup35, or Mitotic phosphoprotein 44, is a nuclear protein known to be present on the nuclear pore complex and is tightly associated with the nuclear membrane and lamina. This RNA-associated protein belongs to the Nup53 family and has a MPPN (mitotic phosphoprotein N' end) domain. It has a small number of scattered phenylalanine-glycine repeats, but not the large domains seen in other nucleoporins. Though the exact function of NUP35 is yet to be revealed, it may function as a component of the nuclear pore complex (NPC) which are collectively referred to as nucleoporins (NUPs). It also may play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors, and may also play an important part in the association of MAD1, a transcriptional repressor with the NPC. NUP35 is widely expressed in most of the tissues.
Catalog #N7100-42A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlLiver lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa280-330 of human NUP35.
SpecificityRecognizes human NUP35. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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