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You are here:Home » Antibodies » Abs to Enzymes, Nucleotidase » Anti -5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

Anti -5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B FC
CD73 (Ecto-5'nucleotidase, 5 '-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.
Catalog #N7100-71B
ApplicationsSuitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml .Detection limit for rmCD73 is
~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. Detection limit for rmCD73 is ~1ng/lane under non-reducing and reducing conditions.
Flow Cytometry (Murine splenocytes): 25ug/ml. Add 100ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not excedding 200ul.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenCHO cell-derived rmCD73. (aa 29-549)
SpecificityRecognizes mouse CD73.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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