Anti -5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E,
NTE, Purine 5 Prime Nucleotidase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| CD73 (also known as [ecto]-5'-nucleotidase/5'-NT) is a variably glycosylated, 69-73kD member of the 5'-nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3 Treg lymphocytes, FDCs, and B cells. CD73 hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human CD73 is a 523aa GPI-linked protein (aa27-549). It contains a large Zn-dependent nucleotidase catalytic region (aa28-532) and a C-terminal substrate binding site (aa500-506). On the cell surface, it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa405-454, and a second possesses a 12aa substitution for aa253-574. Over aa1-511, human CD73 shares 88% aa identity with both mouse and rat CD73. | | | Catalog # | N7100-71D | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1,000-1:2,000 | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 10B1959 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant human CD73 (aa27-252) purified from E. coli. | | Specificity | Recognizes human CD73. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | 5'-Nucleotidase, Ecto (CD73) |
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