Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Nucleotidase » Anti -5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase) discontinued see N7100-71A

Anti -5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E,
NTE, Purine 5 Prime Nucleotidase) discontinued see N7100-71A

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
CD73 (also known as [ecto]-5'-nucleotidase/5'-NT) is a variably glycosylated, 69-73kD member of the 5'-nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3 Treg lymphocytes, FDCs, and B cells. CD73 hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human CD73 is a 523aa GPI-linked protein (aa27-549). It contains a large Zn-dependent nucleotidase catalytic region (aa28-532) and a C-terminal substrate binding site (aa500-506). On the cell surface, it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa405-454, and a second possesses a 12aa substitution for aa253-574. Over aa1-511, human CD73 shares 88% aa identity with both mouse and rat CD73.
Catalog #N7100-71D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000-1:2,000
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No10B1959
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human CD73 (aa27-252) purified from E. coli.
SpecificityRecognizes human CD73.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names5'-Nucleotidase, Ecto (CD73)


External Links