Anti -5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E,
NTE, Purine 5 Prime Nucleotidase) (PE)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| CD73 (also known as [ecto]-5’-nucleotidase/5’-NT) is a variably glycosylated, 69-73kD member of the 5’-nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3 Treg lymphocytes, FDCs, and B cells. CD73 hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human CD73 is a 523aa GPI-linked protein (aa27-549). It contains a large Zn-dependent nucleotidase catalytic region (aa28-532) and a C-terminal substrate binding site (aa500-506). On the cell surface, it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa405-454, and a second possesses a 12aa substitution for aa253-574. Over aa1-511, human CD73 shares 88% aa identity with both mouse and rat CD73. | | | Catalog # | N7100-71F | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Hybridoma | Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, CHO-derived, recombinant human 5’ Nucleotidase/CD73. | | Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 10K16 | | Host | Mouse | | Source | Human | | Concentration | 100 Tests/ml | | Form | Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE). | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | CHO-derived, recombinant human 5’ Nucleotidase/CD73 (aa1-511; Accession #AAH65937). | | Specificity | Recognizes human CD73. Species sequence homology: mouse, rat (88%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
