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You are here:Home » Antibodies » Abs to RNA Processing » Anti -Nudix-type Motif 21 (NUDT21, Nucleoside Diphosphate Linked Moiety X-type Motif 21, Cleavage and Polyadenylation Specific Factor 5, CFIM25, CPSF5, DKFZp686H1588)

Anti -Nudix-type Motif 21 (NUDT21, Nucleoside Diphosphate Linked Moiety X-type Motif 21,
Cleavage and Polyadenylation Specific Factor 5, CFIM25, CPSF5, DKFZp686H1588)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC FC
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.
Catalog #N7400-25
ApplicationsSuitable for use in Flow Cytometry, Dot Blot, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 0.5-2ug/sample. HeLa cells were fixed in PBS, 2% paraformaldehyde and then permeabilized in 90% methanol prior to staining.
Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug/sample
Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS prior to immunostaining.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No8C255
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartial sequence of full-length recombinant human Nudix-type Motif 21
SpecificityRecognizes human Nudix-type Motif 21. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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