| Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs and other organic anions from the blood by a process called “renal secretion.” Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway and placenta. Several multispecific OATs (OAT1-3, OAT-K1, OATK2) and OATPs (oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). OAT2, previously called NLT (novel liver-specific transporter) shows 42% homology with OAT2. OAT2 (rat 535 aa; human 548 aa) is predominantly expressed in liver, and to a lower level in kidney. OAT2 mediates sodium-independent, multispecific organic anion transport (PAH, salicylate and acetylsalicylate, prostaglandin E2, and dicarboxylate). |
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| Catalog # | O3001-09 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | ELISA: 0.5-1ug/ml. Control peptide can be used to coat plate at 1ug/ml. |
| Western Blot: 1-10ug/ml using ECL. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | O3001-05: OATP2, Control Peptide, Rat |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% BSA. |
| Purity | Purified by affinity chromatography. |
| Immunogen | A synthertic peptide corresponding to 15aa near the N-terminus, extracellular domain of rat OATP2 (035913), conjugated to KLH. Species Sequence Homology: mouse; 85%, human; 78%. |
| Specificity | Recognizes rat OATP2 (Organic Anion Transporting Polypeptide 2). No significant sequence homology is detected with other OATs or other proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
