| Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Recently OAT3 (rat 536-aa, human 568 aa) has been cloned that is most closely related to OAT1 (~49% identity). Rat OAT3 is expressed in the liver, brain, kidney and eye. OAT3 also mediates Na-independent uptake of several organic anions (PAH, ochratoxin, and estrone sulfate, etc). OAT3 shows 92% homology with mouse Roct (reduced in osteosclerosis transporter), possibly an ortholog of rat OAT3. As compared to OAT1-2, strong expression of OAT3 in brain suggests its role in removing organic anion in the brain. |
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| Applications: |
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| Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. |
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| Catalog # | O3003-20 |
| Recommended Dilution | Western Blot: 1-10ug/ml using ECL. |
| ELISA: 0.5-1ug/ml. Coat with ELISA plates with Control Peptide at 1ug/ml. |
| Immunohistochemistry: 2-20ug/ml |
| Immunofluorescence: 2-20ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | O3003-22 |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as liquid in PBS, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to 16aa near the N-terminus, cytoplasmic domain of rat OAT3 (KLH). |
| Specificity | Recognizes rat OAT3. No significant sequence homology is detected with other OATs or other proteins. Species sequence homology: mouse, 93%; human and rabbit, 87%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
