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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Occludin (HRP)

Anti -Occludin (HRP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
The 65kD occludin protein was first identified in chicken using monoclonal antibodies(11,12). The chicken occludin cDNA was subsequently cloned and sequenced, and the amino acid sequence revealed that the occludin protein is organized into five distinct domains: a short amino terminal cytoplasmic domain (domain A), two extracellular loops (domains B and D) separated by a short intracellular loop (domain C), and a long carboxy-terminal cytoplasmic tail (domain E)(11,12). The C-terminal tail of occludin is required for both for its localization at tight junctions and for its direct interaction with the ZO-1 protein(12). One interesting feature of the occludin protein is that its amino acid sequence has not been highly conserved throughout evolution(13). This fact made isolating the mammalian homologues of chicken occludin a rather difficult task. Recently, however, the sequences of the full length cDNAs encoding occludin of rat-kangaroo, human, mouse, and dog were reported(13). At the amino acid level, the human, murine, and canine occludin proteins are highly homologous (~90% identity); however, the mammalian proteins exhibit a considerable degree of divergence from the rat kangaroo and chicken proteins(13).
Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Catalog #O5200
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G217
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% Proclin, 40% glycerol. Labeled with Horseradish peroxidase (HRP).
PurityPurified by Protein A affinity chromatography.
ImmunogenGST fusion protein consisting of the C-terminal region of human occludin
SpecificityRecognizes human occludin protein. Species Crossreactivity: mouse, rat and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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