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Specifications

Clone Host Grade Applications Monoclonal Rabbit Purified B

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation
of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of
liver cells by hepatitis C virus (HCV) (5).
Catalog #	O5200-01
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	11C1056
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
Purity	Purified
Immunogen	Synthetic peptide corresponding to central residues of human occludin.
Specificity	Recognizes endogenous levels of total human occludin. Species Crossreactivity: monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.