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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IF

Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).
Catalog #	O5202
Applications	Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions	ELISA: 0.1-1ug/ml
	Western Blot: 0.1-1ug/ml
	Immunofluorescence: 2-3ug/ml
	Optimal dilutions to be determined by the researcher.
Immunofluorescence Protocol	1. Incubate sample in ethanol for 30 minutes at 4°C
	2. Incubate the samples an additional 3 minutes at RT
	in acetone that had been stored at -20°C.
	3. Block the samples then incubate with 2-3ug/ml of O5205 overnight at 4ºC.
	4. Incubate with IgG (FITC) goat anti-mouse for 1 hour at RT.
Recommended secondary antibodies	I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
	I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo
Positive Control	T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma).
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3G217
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
Specificity	Recognizes mammalian Occludin Protein. Species Crossreactivity: human, mouse, rat and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.