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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Occludin

Anti -Occludin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).
Catalog #O5202
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 0.1-1ug/ml
Immunofluorescence: 2-3ug/ml
Optimal dilutions to be determined by the researcher.
Immunofluorescence Protocol1. Incubate sample in ethanol for 30 minutes at 4°C
2. Incubate the samples an additional 3 minutes at RT
in acetone that had been stored at -20°C.
3. Block the samples then incubate with 2-3ug/ml of O5205 overnight at 4ºC.
4. Incubate with IgG (FITC) goat anti-mouse for 1 hour at RT.
Recommended secondary antibodiesI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo
Positive ControlT84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G217
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenGST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
SpecificityRecognizes mammalian Occludin Protein. Species Crossreactivity: human, mouse, rat and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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