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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Occludin (FITC)

Anti -Occludin (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).
Catalog #O5203
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: Detects a band at ~65kD.
Immunofluorescence: 2-10ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlT84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G217
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, 50% glycerol. Labeled with FITC.
PurityPurified by Protein A affinity chromatography.
ImmunogenGST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
SpecificityRecognizes human Occludin Protein. Species Crossreactivity: mouse, rat and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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