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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Occludin

Anti -Occludin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IF
Catalog #O5204
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunofluorescence: 4-20ug/ml
Immunoprecipitation: ~5ug/IP reaction
Immunohistochemistry (FFPE): 2-5ug/ml; Protease pretreatment of sections is required prior to staining. Dilute protease to 1-2mg/ml and incubate at 37°C for 10 minutes. Other enzyme and HIER pretreatment methods may not be suitable for this antibody.
Optimal dilutions to be determined by the researcher.
Recommended IF fixation and staining protocol1.“Pre-extract” the cells on ice for 2 min with an ice-cold buffer containing 0.2% Triton X-100.
2. Fix cells in 3% paraformaldehyde in PBS for 30 min at RT.
3. Permeabilize sample with 0.05% Triton X-100 in PBS for 5 min on ice.
4. Incubate samples with the desired concentration of O5204 (4-20ug/ml) on ice for 1 hour.
5. Incubate with IgG (FITC) Pab Gt x Rb for 30 min at RT.
Recommended Secondary AntibodiesI1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
I1904-46N: IgG, H&L (FITC) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenFusion protein containing the C-terminal 150aa of human occludin.
SpecificityRecognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species Crossreactivity: mammalian, mouse, rat and canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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