Oct4, Human, Primer Pair (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1,
HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type
Homeodomain-containing DNA-binding Protein)
Pricing
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| Human Oct3/4 (POU domain class 5 transcription factor 1; POU5F1) specific PCR primers for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression. | | | Catalog # | O5403-01F | | Components | Primer Pair: Lyophilized. Each vial contains 375 pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25°C; 0.1mM EDTA, pH 8.0 @ 25°C). | | Positive Control 55 | Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes. | | GenBank Accession Number | NM_002701 | | Product Sizes | cDNA: 486 base pairs (bp) | | Genomic DNA: 1031 bp product predicted and observed. Product observed from pseudogene at 486 bp. Treatment of RNA with RNase-free DNase is recommended to remove contaminating genomic DNA. | | Positive Control 55: 320 bp | | Alternate splice variants: Amplifies both variants to give the same size product | | Suggested Reaction Conditions | For each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment. | | Thermocycling Conditions | A typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC. | | Storage and Stability | The Primer Pair resuspended in water or TE buffer is stable for up to one year at -20°C in a non-frost free freezer. Aliquot in single use portions. Do not use past the expiration date above. Avoid repeated freeze-thaw cycles. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Oct4, Human, Primer Pair (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein) |
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