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You are here:Home » Antibodies » Abs to Enzymes, Glycosyltransferase » Anti -OGT, ID (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase Subunit p110)

Anti -OGT, ID (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD
Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase
Subunit p110)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the 2 processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The predicted 920aa human protein contains 9 tetratricopeptide repeats and a putative bipartite nuclear localization signal. Southern blot analysis indicated that a single OGT gene is present in human.
Catalog #O5750-01B
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:64,000
Western Blot: 1:64,000 & 0.05-0.2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-YEHPKDLKLSDGR from an internal region of human OGT.
SpecificityRecognizes human OGT. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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