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Anti -OGT, ID (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase Subunit p110)
Anti -OGT, ID (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase 110kD
Subunit, O-linked N-acetylglucosamine Transferase 110kD Subunit, O-GlcNAc Transferase
Subunit p110)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the 2 processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The predicted 920aa human protein contains 9 tetratricopeptide repeats and a putative bipartite nuclear localization signal. Southern blot analysis indicated that a single OGT gene is present in human. | | | Catalog # | O5750-01B | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:64,000 | | Western Blot: 1:64,000 & 0.05-0.2ug/ml | | Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-YEHPKDLKLSDGR from an internal region of human OGT. | | Specificity | Recognizes human OGT. Species sequence homology: Canine, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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