USBio Logo

O6350 Mouse Anti-Omi, HtrA2 (HtrA serine peptidase 2)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
O43464
Isotype
IgG1
Clone Number
196C429
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.

Htra2 is a novel member of the Htra serine protease family. Human Htra2 is localized in the mitochondria and is involved in mammalian stress response pathways. Human Htra2, predominantly a nuclear protease, is upregulated in mammalian cells under stress, due to heat shock or tunicamycin treatment. Htra2 plays a role in regulating apoptosis by binding and inhibiting caspases.
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DAIBLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N-terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.
Applications
Useful for detection of Omi/HtrA2 in cells by Western blot analysis. Jurkat can also be used as a positive control. This mAb does not cross-react with mouse Omi/HtrA2. It can be used in IHC(5). Western blot detection of Omi/HtrA2 in HL-60 cell lysate. Two protein bands of approximate molecular weight of 50 and 38kD were detected at 3ug/ml dilution. The 50kD and 38kD bands may represent precursor and mature form of Omi. IHC testing(5) shows cytoplasmic staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).
Recommended Dilution
Western blot: 2-3ug/ml
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide containing amino acids 335-350 of human Omi/HtrA2.
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 0.2% gelatin.
Purity
Purified by Protein G affinity chromatography.
Specificity
This mAb does not cross-react with mouse Omi/HtrA2.
References
1. Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174. 2. Liston P, Fong WG, and Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80. 3. Vaux DL and Silke J. Mammalian mitochondrial IAP binding proteins. Biochem. Biophys. Res. Comm. 2003; 304:499-504. 4. Suzuki Y, Imai Y, Nakayama H, et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 2001; 8:613-21. 5. Yang QH, Church-Hajduk R, Ren J, et al. Omi/HtrA2 catalytic cleavage of inhibitor of apoptosis (IAP) irreversibly inactivates IAPs and facilitates caspase activity in apoptosis. Genes Dev. 2003; 17:1487-96. 6. Miller, L., et al., Trends Cell Biol. 9: 323-328 (1999). 7. Zumbrunn, J., et al., FABS Lett. 398: 187-192 (1996). 8. Gui-Ying, N., et al., Biochem. J. 371: 39-48 (2003). 9. Miller LK. Trends Cell Biol. 9: 323-328 (1999). 10. Deveraux QL, and Reed JC. Gense Dev. 13: 239-252 (1999). 11. Reed JC and Bischoff JR Cell 102: 545-548 (2000). 12. Hegde R, Srinivasual SM, Zhang J, Wasell R, et al. J. Biol. Chem 277: 432-438 (2002). 13. Sug Hyung Lee, et al. Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer. Apmis Volume 111 ( 5): 586 (2003).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved