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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Omi, HtrA2 (HtrA serine peptidase 2)

Anti -Omi, HtrA2 (HtrA serine peptidase 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.
Catalog #O6350
Htra2 is a novel member of the Htra serine protease family. Human Htra2 is localized in the mitochondria and is involved in mammalian stress response pathways. Human Htra2, predominantly a nuclear protease, is upregulated in mammalian cells under stress, due to heat shock or tunicamycin treatment. Htra2 plays a role in regulating apoptosis by binding and inhibiting caspases.
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DAIBLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N-terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.
ApplicationsUseful for detection of Omi/HtrA2 in cells by Western blot analysis. Jurkat can also be used as a positive control. This mAb does not cross-react with mouse Omi/HtrA2. It can be used in IHC(5). Western blot detection of Omi/HtrA2 in HL-60 cell lysate. Two protein bands of approximate molecular weight of 50 and 38kD were detected at 3ug/ml dilution. The 50kD and 38kD bands may represent precursor and mature form of Omi. IHC testing(5) shows cytoplasmic staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).
Recommended DilutionWestern blot: 2-3ug/ml
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H216
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide, 0.2% gelatin.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide containing amino acids 335-350 of human Omi/HtrA2.
SpecificityThis mAb does not cross-react with mouse Omi/HtrA2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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