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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Omi, HtrA2, CT (HtrA Serine Peptidase 2, High Temperature Requirement Protein A2, OMI, Omi Stress-regulated Endoprotease, PARK13, PRSS25, Serine Protease 25, Serine Protease HtrA2 Mitochondrial, Serine Protease Omi) (Azide Free)

Anti -Omi, HtrA2, CT (HtrA Serine Peptidase 2, High Temperature Requirement Protein A2,
OMI, Omi Stress-regulated Endoprotease, PARK13, PRSS25, Serine Protease 25, Serine
Protease HtrA2 Mitochondrial, Serine Protease Omi) (Azide Free)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Both isoforms of HTRA2 are thought to play a role in cellular stress response as they are up-regulated following heat shock and endoplasmic reticulum stress. They promote cell death by binding and inactivating inhibitor of apoptosis proteins (IAPs), which contain baculoviral IAP repeat (BIR) binding domains. This causes an increase in caspase activity. Isoform 1 (though not 2) can also proteolytically cleave IAPs. Additionally HtrA2 isoforms can induce cell death via a mechanism dependent on its serine protease activity that is independent of IAP and caspases. Defects in HtrA2 are linked to Parkinson disease 13. The isoforms are localized to the intermembrane space of the mitochondria to be released into the cytoplasm in response to apoptotic stimuli. Isoform 1 is ubiquitous while isoform 2 is expressed predominantly in the kidney, colon and thyroid.
Catalog #O6350-01M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml. Detects isoforms 1 and 2 at ~45kD and 35kD in U937 cell lysates
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-terminus of human HtrA2
SpecificityRecognizes two isoforms of human High temperature requirement protein A2 (HtrA2), isoform 1 (a 45kDa protein) and isoform 2 (a 35kDa protein).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.4.21.108, HtrA-like Serine Protease, Protease Serine 25, Serine Protease HtrA2 Mitochondrial Precursor


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