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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1)

Anti -Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4
Oncofetal Trophoblast Glycoprotein, M6P1)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72kD glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420aa in length. It contains a 324aa extracellular region (aa32-355) that shows one Ser-rich region followed by seven Leu-rich repeats (aa90-355). Over aa31-355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.
Catalog #O6450-01
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 1ug/ml
Immunohistochemistry: 3-10ug/ml detects 5T4 in cells and tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa31-355 from the extracellular domain of human Oncofetal Antigen 5T4, expressed in NSO cells.
SpecificityRecognizes the extracellular domain of human Oncofetal Antigen 5T4 at ~72kD. Shows ~20% crossreactivity with mouse 5T4. Species Sequence Homology: Mouse (81%), canine (85%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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