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Specifications

Catalog #	O6500-01
Source	Recombinant corresponding to 227aa human Oncostatin M expressed in E. coli.
Molecular Weight	~26kD
Endotoxin	~0.1ng/ug
Biological Activity	ED50 <2ng/ml ( > 5x105units/mg). The biological activity is determined by measuring the oncostatin M concentration dependent stimulation of proliferation of TF-1 cells. The suggested concentration range for in vitro activity studies is 0.2-20.0ng/ml. The optimal concentration should be determined for each specific application. Oncostatin M is observed to exert variable, cell type dependent effects. For example, oncostatin M is observed to stimulate the growth of normal fibroblasts and AIDS-related Kaposi sarcoma cells, but is observed to inhibit the growth of A375 melanoma cells.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity	~98% (SDS-PAGE, HPLC)
Concentration	Not determined
Form	Supplied as a lyophilized powder. BSA free. No preservative added. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.