| Oncostatin M (OSM) is a member of the interleukin-6 family of cytokines. Of these cytokines, OSM is closely related structually, genetically and functionally to leukemia inhibitory factor. However, OSM-specific biological activities have been reported in hematopoiesis and liver development. |
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| Catalog # | O6500-01A |
| Description | Recombinant Human OSM produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids and having a molecular mass of 26kD. Recombinant OSM is purified by proprietary chromatographic techniques. |
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Ile-Gly |
| Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
| Biological Activity | Human Oncostatin M is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5 x 105 IU/mg. |
| Endotoxin | 0.1ng/ug (IEU/ug) of recombinant OSM. |
| Protein Content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280 nm using the absorbency value of 0.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| 2. Analysis by RP-HPLC, using a standard solution of OSM as a Reference Standard. |
| Solubility | It is recommended to reconstitute the lyophilized rHuOSM in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Recombinant Human OSM although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution OSM should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Molecular Weight | 26kD |
| Source | E. coli |
| Purity | 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder without additives. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
