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Anti -Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor, KOR, KOR-1, OPRK)
Anti -Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor,
KOR, KOR-1, OPRK)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
IH IC IF |
|
| Catalog # | O7030-25 | | Applications | Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: 1:250-1:500 Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) 4% paraformaldehyde fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. | | Immunofluorescence: Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at RT. O7030-25 was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with O7030-25. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. | | Immunocytochemistry: 1:500 Kappa opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to residues 366-380 of the carboxy-terminus of human kappa opioid. | | Specificity | Recognizes human opioid receptor, kappa. Species crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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