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You are here:Home » Antibodies » Abs to Opioid Receptors, Agonists » Anti -Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor, KOR, KOR-1, OPRK)

Anti -Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor,
KOR, KOR-1, OPRK)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH IC IF
Catalog #O7030-25
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:250-1:500 Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) 4% paraformaldehyde fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Immunofluorescence: Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at RT. O7030-25 was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with O7030-25. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
Immunocytochemistry: 1:500 Kappa opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.
PuritySerum
ImmunogenSynthetic peptide corresponding to residues 366-380 of the carboxy-terminus of human kappa opioid.
SpecificityRecognizes human opioid receptor, kappa. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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