Anti -Opioid Receptor 1, mu, aa384-398 (MOR1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IH IF |
|
| Most actions of exogenous opioids, such as morphine, are mediated through the mu-opioid receptor, including analgesia, tolerance and reward. In general, opioids modulate numerous central and peripheral processes including pain perception, neuroendocrine secretion and the immune response. The opioid signal is transduced from receptors through G proteins to various different effectors. Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal. For example, activation of opioid receptors increases phosphatidyl-inositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities. | | | Catalog # | O7030-40 | | Applications | Suitable for use in Immunofluorescence, Immunohistochemistry, and Western Blot. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry: 1:500-1:1000 (Cy3 technique); 1:6000-1:10,000 (Biotin/avidin-HRP technique). | | Dilute antibody in PBS, 0.3% Triton X-100. Incubate 18-24 hrs at 2-8°C. Perfusion Fixation: With 500ml 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 20-30 minutes. Post Fixation: 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 1.5 hours at 4°C. Sections: 10um cryostate. Note that a change in the fixation or buffering system as used in our protocol may change the configuration of the protein, and therefore, may alter the reactivity with the tissue tested. Pre-adsorption with immunogen peptide at 10ug/ml completely eliminates labeling. | | Optimal dilutions to be determined by the researcher. | | Control Peptide | O7030-40A | | Control Tissues | rat caudate putamen and spinal cord (dorsal horn) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid with 40% glycerol. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to aa384-398 (NHQLENLEAETAPLP) from the C-terminus of the cloned rat mu opioid receptor-1 (bovine thyroglobulin coupled by gluteraldehyde). | | Specificity | Mu opioid receptor, C-terminal domain. Specificity confirmed by immunolabeling of transfected cells, Western blot analysis and immunoisolatin stuies. No reactivity to the N-terminus, second extracellular loop or a different segment of the carboxy tail of the mu opioid receptor. No reactivity to either the delta or kappa opioid receptor. Species crosseactivity: Human, rat and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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