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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -ORAI1 (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)

Anti -ORAI1 (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium
Channel Protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels (1 for review). ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry (2). It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor (3). ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE.
Catalog #O7044
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlWB: Human ovary tissue lysate
IHC: Human spleen tissue
Control PeptideO7044-01: ORAI1, Human, Control Peptide (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide correspoding to 18aa from near the amino terminus of human ORAI1 (GenBank Q96D31).
SpecificityRecognizes human ORAI1. Predicted to have no crossreactivity to ORAI2 or ORAI3. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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