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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -ORAI1, CT (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)

Anti -ORAI1, CT (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium
Channel Protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.
Catalog #O7044-03
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml, detects a band of approximately 49kD in 49 cell lysates.
Optimal dilution to be determined by researcher.
Recommended Positive ControlHuman Ovary Total Protein Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquidi in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography
ImmunogenA 16 amino acid peptide from near the carboxy terminus of human ORAI1.
SpecificityRecognizes human ORAI1. Species Crossreactivity: mouse, rat. Not expected to cross react with either ORAI2 or ORAI3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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