You are here:Home
»
Antibodies
»
Abs to Membrane Proteins
»
Anti -ORAI1, CT (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)
Anti -ORAI1, CT (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium
Channel Protein 1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry. | | | Catalog # | O7044-03 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml, detects a band of approximately 49kD in 49 cell lysates. | | Optimal dilution to be determined by researcher. | | Recommended Positive Control | Human Ovary Total Protein Lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Concentration | ~1mg/ml | | Form | Supplied as a liquidi in PBS, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography | | Immunogen | A 16 amino acid peptide from near the carboxy terminus of human ORAI1. | | Specificity | Recognizes human ORAI1. Species Crossreactivity: mouse, rat. Not expected to cross react with either ORAI2 or ORAI3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
