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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.
Catalog #	O7500-05
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000-1:1000. Use 50-100ng of control per well.
	Western Blot: 1-10ug/ml (ECL)
	Optimal dilution to be determined by the researcher.
Control Peptide	O7500-10: Orexin A, Mouse, Control Peptide (Hypocretin A).
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Purity	Purified by antigen affinity chromatography.
Immunogen	Synthetic peptide, 18aa of mouse Orexin A (KLH coupled). Epitope
Specificity	Recognizes the full-length (1-33 aa) of mouse Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130 aa), the anti-Orexin A antibodies may also react with prepro orexins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.