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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Orexin A (Hypocretin A)

Anti -Orexin A (Hypocretin A)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.
Catalog #O7500-05
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:1000. Use 50-100ng of control per well.
Western Blot: 1-10ug/ml (ECL)
Optimal dilution to be determined by the researcher.
Control PeptideO7500-10: Orexin A, Mouse, Control Peptide (Hypocretin A).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
PurityPurified by antigen affinity chromatography.
ImmunogenSynthetic peptide, 18aa of mouse Orexin A (KLH coupled). Epitope
SpecificityRecognizes the full-length (1-33 aa) of mouse Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130 aa), the anti-Orexin A antibodies may also react with prepro orexins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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