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O7505-11V Goat Anti-Orexin Receptor Type 2 (HCRTR2, Ox-2-R, Ox2-R, Ox2R, Hypocretin Receptor Type 2)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001517.1
Gene ID
3062, 25605
Shipping Temp
Blue Ice
Storage Temp
-20°C

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:128,000. Western Blot: 1-3ug/ml, observed in human brain (cerebral cortex, hippocampus and substantia nigra) lysates on ~50kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to SGTKLEDSPPCRN, from human HCRTR2, at N-terminal. Species sequence homology: Bovine, canine and porcine.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human HCRTR2.
References
1. Spinazzi R, Rucinski M, Neri G, Malendowicz LK, Nussdorfer GG. Preproorexin and orexin receptors are expressed in cortisol-secreting adrenocortical adenomas, and orexins stimulate in vitro cortisol secretion and growth of tumor cells. J Clin Endocrinol Metab. 2005 Jun;90(6):3544-9. Epub 2005 Mar 29.
USBio References
No references available
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