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Abs to Osteocalcin
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Anti -Osteocalcin (Bone-Gla-Protein, BGP)
Anti -Osteocalcin (Bone-Gla-Protein, BGP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
E B |
|
| Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone. | | | Catalog # | O8060-17 | | This antibody can be used effectively for research of metabolic bone disease and ectopic calcification. | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA: 1:1000 (2ug/ml) | | Western Blot: 2-10ug/ml | | Optimal dilutions to be determined by the researcher. | | Hybridoma | P3U1 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG3 | | Clone No | 3G219 | | Host | Mouse | | Source | Bovine | | Concentration | ~2mg/ml | | Form | Supplied as a lyophilized powder from 20mM TBS, pH 7.5, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile ddH2O. | | Purity | Purified by ammonium sulfate fractionation and ion exchange chromatography. | | Immunogen | Bovine Osteocalcin | | Specificity | Recognizes gamma-carboxylated residues 21-31of bovine Osteocalcin (does not react with heat decarboxylated Osteocalcin). | | Species Crossreactivity | Human | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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