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You are here:Home » Antibodies » Abs to Osteocalcin » Anti -Osteocalcin (Bone-Gla-Protein, BGP)

Anti -Osteocalcin (Bone-Gla-Protein, BGP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B
Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone.
Catalog #O8060-17
This antibody can be used effectively for research of metabolic bone disease and ectopic calcification.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:1000 (2ug/ml)
Western Blot: 2-10ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaP3U1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG3
Clone No3G219
HostMouse
SourceBovine
Concentration~2mg/ml
FormSupplied as a lyophilized powder from 20mM TBS, pH 7.5, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile ddH2O.
PurityPurified by ammonium sulfate fractionation and ion exchange chromatography.
ImmunogenBovine Osteocalcin
SpecificityRecognizes gamma-carboxylated residues 21-31of bovine Osteocalcin (does not react with heat decarboxylated Osteocalcin).
Species CrossreactivityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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