Anti -Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein,
Gamma Carboxyglutamic Acid-containing Protein, PMF1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1-2% of the total bone protein. It is a 49aa single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three g carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. | | | Catalog # | O8060-17A | | Applications | Suitable for use in sandwich ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-10ug/ml under reducing or non-reducing conditions. | | Immunohistochemistry (Frozen, paraffin): 1-10ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 9A87 | | Host | Mouse | | Source | Bovine | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 1% BSA, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Full length bovine Osteocalcin. Cellular Localization: Secreted | | Epitope | Includes the gamma-carboxylated residue at position 17. | | Specificity | Recognizes bovine Osteocalcin. | | Species Crossreactivity | Human, rat, canine, chicken, goat, porcine, rabbit, sheep. Does not crossreact with mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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