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You are here:Home » Antibodies » Abs to Osteocalcin » Anti -Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein, Gamma Carboxyglutamic Acid-containing Protein, PMF1)

Anti -Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein,
Gamma Carboxyglutamic Acid-containing Protein, PMF1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1-2% of the total bone protein. It is a 49aa single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three g carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.
Catalog #O8060-17A
ApplicationsSuitable for use in sandwich ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsSandwich ELISA: 1:100-1:2000
Western Blot: 1-10ug/ml under reducing or non-reducing conditions.
Immunohistochemistry (Frozen/paraffin): 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9A87
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 1% BSA
PurityPurified by Protein A affinity chromatography
ImmunogenFull length bovine Osteocalcin. Cellular Localization: Secreted
EpitopeIncludes the gamma-carboxylated residue at position 17.
SpecificityRecognizes bovine Osteocalcin.
Species CrossreactivityHuman, rat, canine, chicken, goat, porcine, rabbit, sheep. Does not crossreact with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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