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You are here:Home » Antibodies » Abs to Osteocalcin » Anti -Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein, Gamma Carboxyglutamic Acid-containing Protein, PMF1)

Anti -Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein,
Gamma Carboxyglutamic Acid-containing Protein, PMF1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Osteocalcin, also known as Bone-Gla-Protein (BGP), is a single chain (Mr=5800) vitamin K dependent protein produced by osteoblasts. It is found in high concentrations in bone (1-4). Post-translational modification by a vitamin K dependent carboxylase produces three g-carboxyglutamic acid residues at positions 17, 21 and 24. The mature protein contains 49 amino acids and a single intrachain disulfide bond joining Cys23 to Cys29. The secondary structure is highly calcium dependent and contains 14% alpha-helix, 20% beta-sheet and 67% random form in the presence of calcium, and 1% alpha-helix, 20% beta-sheet and 79% random form in the absence of calcium (4). BGP binds to phospholipid vesicles in the presence of calcium ions (Kd=6 x 10e-6 M). BGP also binds hydroxyapatite and is an efficient inhibitor of hydroxyapatite seeded crystal growth, suggesting a regulatory role in bone mineralization. Although the fundamental role of BGP in vivo remains elusive, in vitro experimentation suggests roles in both bone resorption and bone formation. BGP has been shown to function as a chemo-attractant in an in vitro macrophage mediated bone resorption system. The serum concentration of BGP has been linked to bone formation rates, and BGP levels are currently used in research assessment of osteoporosis and other metabolic bone disease states.
Catalog #O8060-17C
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA (sandwich): 1-10ug/ml
Western Blot: 1-10ug/ml. Reducing or non-reducting conditions possible.
Immunohistochemistry (paraffin, frozen): 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Cellular Localization Secreted
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9A88
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 1% BSA. No preservative added.
PurityPurified
ImmunogenBovine osteocalcin.
SpecificityRecognizes bovine Osteocalcin.
Species CrossreactivityHuman, chicken, canine, goat, porcine, rabbit, sheep. Does not crossreact with Mouse or Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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