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You are here:Home » Antibodies » Abs to Osteocalcin » Anti -Osteocalcin (Bone-Gla-Protein, BGP)

Anti -Osteocalcin (Bone-Gla-Protein, BGP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B
This antibody can be used effectively for research of metabolic bone disease and ectopic calcification (5).
Catalog #O8060-18
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:3000 (0.67ug/ml)
Western Blot: 2-10ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine Osteocalcin
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G220
HostMouse
SourceBovine
Concentration~2mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile distilled water or sterile 40-50% glycerol.
PurityPurified by ammonium sulfate fractionation and ion exchange chromatography.
ImmunogenBovine Osteocalcin.
SpecificityRecognizes bovine gamma-carboxylated residues 15-31. Does not react with heat de-carboxylated Osteocalcin. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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