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You are here:Home » Antibodies » Abs to Osteonectin » Anti -Osteonectin (SPARC, Secreted Protein, Acidic, Cysteine-rich, ON, OSN)

Anti -Osteonectin (SPARC, Secreted Protein, Acidic, Cysteine-rich, ON, OSN)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH FC
SPARC, also known as Osteonectin and basement membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage and morphogenetic processes such as modeling of extracellular matrix.
Catalog #O8063-12
ApplicationsSuitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml
Flow Cytometry: 3-10ug/ml per 10e6 cells.
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to Ala18-Ile302 from mouse Osteonectin expressed in NSO cells (P07214). Species Sequence Homology: rat; 97%, human, canine, bovine; 92%, chicken; 83%
SpecificityRecognizes mouse Osteonectin. Shows <1% crossreactivity with recombinant human Osteonectin in direct ELISAs and Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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