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You are here:Home » Antibodies » Abs to Osteopontin » Anti -Osteopontin, aa59-74 (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1, Nephropontin, Urinary Stone Protein, ETA-1, MGC110940, PSEC0156)

Anti -Osteopontin, aa59-74 (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1,
SPP1, Early T-Lymphocyte Activation 1, Nephropontin, Urinary Stone Protein, ETA-1,
MGC110940, PSEC0156)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
Osteopontin is a single-chain polypeptide with a molecular weight of ~35kD. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.
Catalog #O8064-01J
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:1000
Immunohistochemistry (Paraffin): 1:1:100-1:1500
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute wiht 20ul sterile ddH2O.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa59-74, PSKSNESHDHMDDMDD from human Osteopontin, conjugated to KLH.
SpecificityRecognizes human Osteopontin (aa 59-74).
Species CrossreactivityMouse. No crossreactivity with human Osteonectin, and human Bone Sialoprotein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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