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You are here:Home » Antibodies » Abs to Osteopontin » Anti -Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1

Anti -Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early
T-Lymphocyte Activation 1

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Osteopontin is a 34kD extracellular matrix protein with a cell-binding domain. It was originally identified as a major component of the noncollagenous organic bone matrix however it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. This antibody should prove useful in studying the expression of this matrix glycoprotein in tissue homeostasis.
Catalog #O8064-04B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 2-4ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:25. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells, Gastric carcinoma or Osteosarcoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide derived from internal domain of human Osteopontin. Cellular Localization: Cytoplasmic
SpecificityRecognizes human Osteopontin. MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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