Anti -Osteopontin (SPP1, Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I,
Early T-lymphocyte Activation 1), HGNC:11255, BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted
Phosphoprotein-1 (Osteopontin, Bone Sialoprotein), Secreted Phosphoprotein 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
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| Osteopontin is a single-chain polypeptide with a molecular weight of ~35kD. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect. | | | Catalog # | O8064-07 | | Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. | | Recommended Dilutions | | | ELISA: 1:64,000 | | Western Blot: 1-3ug/ml. An ~30kD band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kD glycoprotein (calculated MW of 33.8kD according to NP_000573.1). | | Optimal dilutions to be determined by the researcher. | | Peptide Blocking | Corresponding peptide is available for Peptide Blocking studies. See O8064-07-P. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide with the sequence of C-DPKSKEEDKHLK from the C-terminus of Osteopontin | | Specificity | Recognizes human Osteopontin/SPP1. Species Sequence Homology: mouse, rat and canine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Osteopontin (SPP1, Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-lymphocyte Activation 1), HGNC:11255, BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted Phosphoprotein-1 (Osteopontin, Bone Sialoprotein), Secreted Phosphoprotein 1) |
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