Anti -Osteopontin (OPN, BNSP, Bone Sialoprotein 1, Bone Sialoprotein I, BSPI, Early T Lymphocyte Activation 1, ETA-1, HGNC:11255, MGC110940, Nephropontin, Secreted Phosphoprotein 1, SPP1, SPP-1, Urinary Stone Protein, Uropontin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
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| Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. | | | Catalog # | O8064-08 | | Applications | Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Peptide ELISA: 1:2000 | | Western Blot: 1:1000, Detects a ~30kD band in Jurkat and MOLT4 cell lysates. This band corresponds to the cleavage product from the 60kD glycoprotein. | | Immunohistochemistry (Paraffin): 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | Human thymus, tonsil and spleen lysates | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to the C-terminus region of human Osteopontin, C-DPKSKEEDKHLK (Accession #NP_000573.1). Cellular Localization: Secreted | | Specificity | Recognizes human Osteopontin. Species Crossreactivity: mouse, rat and canine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Osteopontin (OPN, BNSP, Bone Sialoprotein 1, Bone Sialoprotein I, BSPI, Early T Lymphocyte Activation 1, ETA-1, HGNC:11255, MGC110940, Nephropontin, Secreted Phosphoprotein 1, SPP1, SPP-1, Urinary Stone Protein, Uropontin) |
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