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You are here:Home » Antibodies » Abs to Osteopontin » Anti -Osteopontin (OPN, BNSP, Bone Sialoprotein 1, Bone Sialoprotein I, BSPI, Early T Lymphocyte Activation 1, ETA-1, HGNC:11255, MGC110940, Nephropontin, Secreted Phosphoprotein 1, SPP1, SPP-1, Urinary Stone Protein, Uropontin) (BSA, Azide Free)

Anti -Osteopontin (OPN, BNSP, Bone Sialoprotein 1, Bone Sialoprotein I, BSPI, Early T
Lymphocyte Activation 1, ETA-1, HGNC:11255, MGC110940, Nephropontin, Secreted
Phosphoprotein 1, SPP1, SPP-1, Urinary Stone Protein, Uropontin) (BSA, Azide Free)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin.
Catalog #O8064-08X
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsPeptide ELISA: 1:64,000
Western Blot: 1-3ug/ml, Detects a ~30kD band in Jurkat and MOLT4 cell lysates. This band corresponds to the cleavage product from the 60kD glycoprotein.
Immunohistochemistry (Paraffin): 1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Thymus, Tonsil and Spleen lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS. Also available with BSA and azide, see O8064-08.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-DPKSKEEDKHLK of the C-terminus region of human Osteopontin. (Accession #NP_000573.1). Cellular Localization: secreted.
SpecificityRecognizes human Osteopontin. Species Crossreactivity: mouse, rat, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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