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You are here:Home » Antibodies » Antibodies-Osteoprotegerin » Anti -Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)

Anti -Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #O8065-02
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Other applications not tested.
Recommended Dilution  
indirect ELISA: 0.5-2ug/ml. Using 100ul/well, used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.53.0ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: 0.5ug/ml on formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. Incubate overnight at 4ºC. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH of 6.0 sodium citrate buffer is recommended.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2.
PurityPuri ed by Immunoaf nity chromatography.
ImmunogenHighly pure (>98%) recombinant human OPG (human Osteoprotegerin).
SpecificityRecognizes human OPG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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