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Anti -Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)
Anti -Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Catalog # | O8065-02 | | Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Other applications not tested. | | Recommended Dilution | | | indirect ELISA: 0.5-2ug/ml. Using 100ul/well, used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well. | | Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5–3.0ng/lane, under either reducing or non-reducing conditions. | | Immunohistochemistry: 0.5ug/ml on formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. Incubate overnight at 4ºC. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH of 6.0 sodium citrate buffer is recommended. | | Optimal dilution determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. | | Purity | Puri ed by Immunoaf nity chromatography. | | Immunogen | Highly pure (>98%) recombinant human OPG (human Osteoprotegerin). | | Specificity | Recognizes human OPG. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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