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You are here:Home » Antibodies » Antibodies-Osteoprotegerin » Anti -Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF) (Biotin)

Anti -Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF) (Biotin)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 amino acid with molecular weight of 55kD as a monomer and 110kD as a disulfide-linked dimer. Osteoprotegerin acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.
Catalog #O8065-03
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionDirect ELISA: 0.25-1ug/ml (100ul/well) Detects at least 0.2-0.4ng/well of recombinant hOPG when used with compatible secondary reagents.
Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant hOPG is 1.5-3.0ng/lane, under either reducing or non-reducing conditions when used with compatible secondary reagents.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.1% BSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder in PBS, pH 7.2. Labeled with Biotin. Reconstitute with 500ul 40-50% glycerol and sterile PBS containing 0.1% BSA.
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant hOPG (human OPG).
SpecificityRecognizes recombinant hOPG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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