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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Ovalbumin (Egg Albumin, Plakalbumin, Allergen Gal d II, Allergen Gal d 2, SERPINB14)

Anti -Ovalbumin (Egg Albumin, Plakalbumin, Allergen Gal d II, Allergen Gal d 2, SERPINB14)

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Specifications

Clone Host Grade Applications
Polyclonal Rat Serum E B
Chicken egg albumin (Ovalbumin) is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylatedglycoprotein. From the aa sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7kD. This sequence completely agrees with the reported m-RNA (messenger RNA) sequence. The carbohydrate and phosphate portions account for an additional 1428 and 160 grams per mole respectively, giving a total molecular weight of 44.3kD. Differential scanning calorimetry indicates the chicken egg albumin denatures at 84°C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following aas: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.
Catalog #O8075-10D
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 (Chemiluminescence)
ELISA: 1:10,000-1:50,000 (using 50-100ng control antigen/well).
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRat
SourceChicken
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.
PuritySerum
ImmunogenHighly purified, antigen grade ovalbumin from chicken egg white.
SpecificityRecognizes chicken egg ovalbumin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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