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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IH Gel

Ovalbumin is a glycoprotein and the major protein of egg white. It has a molecular weight of 43,000. Ovalbumin is used for conjugation or immunological determinations and drug and pharmaceutical processing.
Catalog #	O8075-25
Applications	Suitable for use in Immunoblotting (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Other applications have not been tested.
Recommended Dilution	ELISA: 1:1000-1:3000. Ovalbumin (1ug) was used in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT.
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Chicken
Concentration	~10mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP)
Purity	Puri ed by delipidation, salt fractionation and ion exchange chromatography.
Immunogen	Ovalbumin (Hen Egg White)
Specificity	Recognizes chicken ovalbumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as puri ed and partially puri ed Ovalbumin [Hen Egg White].
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.