| Ovalbumin (OA) from chicken egg white, in which it makes up about 60% of solids. OA is a globular monomer of molecular mass 45kD (1). |
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| Catalog # | O8075-30 |
| Applications | Suitable for use in ELISA and Immunoprecipitation. Not suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken-HRP Rb x Ch |
| I1906-12 IgY, Chicken-FITC Rb x Ch |
| I1906-14 IgY, Chicken-AP Rb x Ch |
| Hybridoma | X63-Ag8.653 myeloma cells with spleen cells from female Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 6F157 |
| Host | Mouse |
| Source | Chicken |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, containing 0.09% sodium azide |
| Purity | Purified by Protein A/G affinity chromatography from culture supernatant |
| Immunogen | Purified chicken ovalbumin adsorbed onto aluminum hydroxide gel |
| Specificity | Specific for native chicken ovalbumin (2). Cross-reactivity with egg albumin from other species has not been tested. Epitope specificity differs from those of clones 6F158 and 6F159. Shows high affinity for OA in its native form, but does not recognize denatured OA. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
