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You are here:Home » Antibodies » Abs to Albumin » Anti -Ovalbumin, Denatured

Anti -Ovalbumin, Denatured

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Ovalbumin (OA) is isolated from chicken egg white in which it makes up about 60% of solids. Native ovalbumin is a globular monomer of molecular mass 45kD.
Catalog #O8075-36
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:12000. (OA antigen coated on plate at 10ug/ml.)
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Culture MediumRPMI 1640 with 10% fetal calf serum
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No6F163
HostMouse
SourceChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenOvalbumin isolated from chicken egg white, formaldehyde treated, and heat denatured by incubation for 1 hr at 110°C.
SpecificityRecognizes denatured chicken ovalbumin (OA). Reacts with OA in denatured or modified form (surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA with OA directly coated onto the microtiter well. In Dot Blot reacts with native OA adsorbed onto nitrocellulose. In Western Blot (overnight) after SDS-PAGE, reacts with OA in both reduced and non-reduced forms. Epitope differs from O8075-34 and O8075-35 due to lack of mutual inhibition of antigen binding.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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