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You are here:Home » Culture Media » Media-Ingredients » Oxgall (Bovine bile, Bacteriological ox bile)

Oxgall (Bovine bile, Bacteriological ox bile)

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Specifications

Oxgall is a purified, dried form of ox bile used in bacteriological media formulations for gastro-intestinal tract organisms, eg, enterocossel agar. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens. Oxbile is also found in Littman Agar, a selective fungal medium.
Catalog #O8175
Oxbile is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids.
Appearance Greenish to light beige or brown, homogeneous powder
Solubility (2%) Gold/greenish to amber, clear, complete
pH (2%) 7.0-8.5
Loss on Drying: 6%
Total Bile Acids 50%
Storage and StabilityStore powdered media at 4°C.Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. Protect from moisture and light by keeping container tightly closed.
CAS Number8008-63-7
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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