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You are here:Home » Antibodies » Antibodies-Enzymes, Oxidase » Anti -P4HA1, ID (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

Anti -P4HA1, ID (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1,
Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Catalog #P0750-50B
ApplicationsSuitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 5ug/ml
Western Blot: 1:32000; 0.1ug/ml
ELISA: 1:32000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-DFARKDEPDAFKE from the internak region of human P4HA1. Species Sequence Homology: mouse, rat, hamster and zebrafish
SpecificityRecognizes human P4HA1. Species Crossreactivity: monkey, bat, bovine, canine, equine, porcine, rabbit and chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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