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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -P4HA1 (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

Anti -P4HA1 (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1,
Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Catalog #P0750-50C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.1ug/ml, observed in lysates of cell lines HEK293, A431 and NIH3T3 on ~60kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-DFARKDEPDAFKE, from P4HA1, at the internal region of the protein (NP_000908.2, NP_001017962.1).
SpecificityRecognizes human P4HA1. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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