Anti -p15INK4b (MTS2, Mitotic Inhibitor/Suppressor Protein, Cyclin-dependent Kinase
Inhibitor, Cyclin-Dependent Kinase 4 Inhibitor B, p14-INK4b, p15-INK4b, Multiple Tumor
Suppressor 2, MTS-2, CDKN2B)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| p15INK4b is a specific inhibitor of cdk4/cdk6 and is periodically expressed during cell cycle. Reportedly, p15INK4b expression is induced approximately 30-fold in human keratinocytes by the treatment with TGF-beta, suggesting that p15INK4b may act as an effector of TGF-b-mediated cell cycle arrest. p15INK4b is mapped to chromosome 9p21, a region of frequent loss in a wide variety of cancers. | | | Catalog # | P0999-53E | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-2ug/ml for 2 hours at RT | | Immunoprecipitation: 2ug/mg protein lysate | | Immunohistochemistry (FFPE): 1:50 for 30 minutes at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at RT for 20 minutes | | Optimal dilutions to be determined by the researcher. | | Positive Control | Colon carcinoma, Hela cells, Ls174T cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | SPM303 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified full-length recombinant protein corresponding to human p15INK4b. Expected Staining Pattern: Nuclear | | Specificity | Recognizes human p15INK4b at ~15kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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