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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein) (BSA & Azide Free)

Anti -p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor
Protein) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH IF
Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.
Catalog #P1000-54X
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation and Immunohistology. Not suitable for Flow Cytometry or Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (native and denatured) : 2ug/ml protein lysate. Co-precipitates p21 proteins.
Immunohistochemistry (formalin and paraffin): 1:25-1:50 for 60 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlLs174T or Raji cells and colon carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.N.488
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also supplied with BSA and sodium azide. See P1000-54.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull length human recombinant p21 protein. MW of Antigen: 21kD
Cellular LocalizationNuclear.
SpecificityRecognizes human p21WAF1/Cip1/Sdi1/Pic1 tumor suppressor protein. Does not react with mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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